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18 Jan 2022 ... SNCA3X 3KO C1, SNCA3X 3KO C2 · General · Donor Information · Ethics · hIPSC Derivation · Culture Conditions · Characterisation · Genotyping · Genetic ...

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All experiments were performed on adult (8–10 weeks) male Cav-3 knockout mice (Cav-3KO) or male wild type littermate controls (WT). Generation and characterization of Cav-3KO mice have been previously described 11. The experiments were performed in adherence with the National Institutes of Health Guidelines on the use of Laboratory Animals ...

We derived haESC3KO with quadruple frameshift mutations at Sfmbt2,Jade1,Smoc1,andGab1usingCRISPR-Cas9-mediated gene editing (D4-haESC3KO)(Figures S1D–S1H). After MII oocyte injectionofD4-haESC3KO,thereconstructedembryos(D4-embry-o3KO) were transferred into the oviduct of pseudopregnant mice (Figure 1E). However, development of D4-embryo3KO was

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